In April, a major review article on so far uncultivated microorganisms was published in the renowned journal Water Research, which was compiled by the entire MultiKulti team. Important uncultivated bacteria and archaea from aquatic habitats were systematically identified. The article can thus serve as a guide for future cultivation initiatives.
Aquatic ecosystems harbor a significant portion of the Earth’s biosphere, yet most microorganisms from these habitats have not been cultured. While recently developed genome-resolved metagenomics and single-cell genomics techniques have underscored the immense genetic breadth and metabolic potential residing in uncultivated Bacteria and Archaea, cultivation of these microorganisms is required to study their physiology via genetic systems, confirm predicted biochemical pathways, exploit biotechnological potential, and accurately appraise nutrient turnover. Over the past two decades, the limitations of culture-independent studies have highlighted the importance of cultivation in bridging this large knowledge gap. This review article highlights the vital contributions of uncultured microbes in aquatic ecosystems, from lakes and groundwater to the surface and depths of the oceans.
Highlights
- Systematic identification of important uncultivated bacteria and archaea.
- Guide for future cultivation initiatives from aquatic ecosystems.
- 84 important lineages relevant for ecosystem services and biotechnology.
- Engineering perspective on cutting edge cultivation strategies.
Publication: Earth’s most needed uncultivated aquatic prokaryotes – ScienceDirect
